Определение и Значение "dark-field microscopy"

What is "dark-field microscopy"?

Dark-field microscopy is a technique used to observe specimens that are difficult to see under normal light conditions. In this method, light is directed at the specimen from the side, rather than from underneath. Only the light that is scattered by the specimen enters the microscope lens, making the specimen appear bright against a dark background. This enhances the visibility of transparent or unstained samples, such as cells or microorganisms, allowing for detailed observation of their structure and movement. Dark-field microscopy is often used in biology and medicine to study living organisms and fine details.